1. Do use things that are considered safe. Humor is a painful thing told playfully, so use humor that rounds on you.
2. Do use a style that you are comfortable with, and have used before.  
3. Do use humor that  relates to your topic.
4. Do be organized and to the point.
5. Do make sure  the story fits your audience.
6. Do speak clearly.
7. Do be aware of the audience’s response. If the joke doesn't work, don’t use it again.
8. Do update the characters in your story to stay current and to relate to your audience.
9. Do use situational humor to get the audience to your point of understanding. 
10. Do stay flexible and be able to rely on anecdotes, epigrams, and exaggerisms when they are usable. 

1. Don’t offend. Stay away from blue humor and wit.
2. Don’t say something a second time that wasn’t funny the first time.
3. Don’t let the story contrast with your speech.
4. Don’t laugh at your own jokes- let the audience decide if they’re funny.
5. Don’t use another comic’s material and attempt to make it look like it’s your original humor. Give credit where credit is due.
6. Don’t use jokes that are not short and to the point.
7. Don’t use old and out-of-date material.
8. Don’t ignore the importance of timing and rhythm in  presentations.
9. Don’t belittle yourself and sacrifice your reputation.

10.  Don’t jump around. Stay on the topic and the audience.

References:  L. Audrieth, Anthony "The Art of Using Humor in Public Speaking" 1998.