Tissue culture

１．General Introduction

２．Ｂasic equipment of the　laboratory

３Manipulation art

４．Component and function of the culture medium

Soilless culture

１．Content introduction

２．The basic way of nonmedium culture

３．The confect and using of the nutrition liquid

４．The examples of soilless culture how a few main plants are do

5.A plan that become homestead to orchard

　　Then had a good laboratory and the basic equipment,must study how to do the manipulation art of tissuc culture,and sklled master the art.

1.Lavation

　　Must have a biger tank for washing containers.Put a rubber cushion in the pool bed for avoid damage the containers.

　　Washerwoman is often be used,and as well as eradicator.If cold washerwoman water wash efficacity is not enough,increase deepness or pyrogenation.There is not need to use chromate-vitriol,because it's efficacity is not good,and often erode,clothings.

　　Befor wash the bottles,must dip in the bottles in the water a little while. Then rinse offf the feculence of the bottles, draining, dip in the bottles, draining, dip in the washerwoman water,along the bottle wall brash up down by bottle brush. then must wash many times,for completed clear away the waserwoman emptyings.The standard of washed bottles: limpidity inside and out side of the bottle wall water film even,can not hang water.Then put the bottles upend or impend for dry by aering. And put the bottles in a over to drying when drying should be slowness warmap.

　　More succus cuvette etc.Use hot washer woman water suction washing then flush by water, lay up rightness for dry by airing. The measure apparatus that with calibrated can not toastde, for avoid glass metamorphosde that may be affect the precision of measure.

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