Recombination Restriction enzymes are mixed with in a test-tube containing the vector molecules, plasmid pBR322. This produces several hundred thousand linear pBR322 molecules each of which has sticky ends complementary to those of the target DNA fragment. Make sure the target DNA is inserted into the tetracycline-resistance conferring gene in pBR322, by choosing a suitable restriction enzyme. The solutions containing the target DNA fragments and the cut pBR322 DNA will be mixed. ATP and DNA ligase will be added. The three main classes of products formed are: foreign DNA fragments which have annealed to themselves. Re-annealed plasmid Recombinant plasmid

Relating Topics
- Transformation
- Identification
- Colony Hybridisation