Sanger Method This method is developed by a British scientist Frederick Sanger. It is used to determine the the nucleotide sequence of DNA molecules. This technique is used by scientist to map the human genome. This method used a modified nucleotide, which terminates the growing DNA chain. Procedures: 4 portions containing the DNA strand to be sequenced prepared. Each portion is given all the ingredients needed for DNA synthesis and in addition, each contains one of the four nucleotides in the modified form. Synthesis of new strand is allowed to take place. The new DNA strands from the 4 portions are separated by electrophoresis. The sequence of newly formed strand can be read from the auto radiograph. This is because by reading what the longest strand terminates with, we would know the base of the first nucleotide. The second longest strand will tell us the base of the second nucleotide and so on.   Relating Topics - Gel Electrophoresis