PCR The Polymerase Chain Reaction (PCR) is a technique used to make copies of a sequence of DNA. It is very fast, and many copies of DNA fragments can be synthesized at a same time.  Procedures: The DNA fragment to be copied is heated slightly to separate the hydrogen bonds in between the two strands.  Nucleotides, ligases and DNA polymerase are added. The mixture is allowed to cool to allow the nucleotides to pair with the complementary bases to produce two strands of DNA complementary to the two strands being copied. This is somewhat similar to DNA replication. At the end of this process, the DNA content doubles. Steps 1-3 is repeated many times to get many copies of the same DNA fragment.

Relating Topics
- Sanger Method