Tranlsate esta paginación

Introduction

Genetic engineering involves the transfer of genes from one organism to an unrelated species. For example, genetic engineers transfer human genes to the bacteria. Then, the human DNA in the plasmids of bacteria will continue to produce the same protein as it did in the human cells. Given suitable nutrients solutions, the bacteria multiply rapidly and produce vast number of offspring. The bacteria reproduce by mitosis and so each daughter bacterium will contain the same DNA and the same plasmids present. The offspring, therefore, form a clone. 

But, before you can insert a segment of DNA into a bacterium, you will need to extract it from a human cell. And there is what this chapter is about. The next chapter DNA recombination will discuss how the wanted gene is inserted into the bacterium.

Generally, there are 3 techniques of getting a gene. They are cutting them by restriction enzymes, reverse transcription and De Novo synthesis. We will look at them separately. A researcher can save the genes in the genomic library and use it as a resource of other genes of interest or for genome mapping.

In the picture, a gene is been inserted into a cell.

Relating Topics
- Vectors
- Stages of Mitosis

Gene Splicing

Table of Contents:
› Introduction
› Restriction enzyme
› Reverse transcription
› De Novo synthesis