| Notes: Watson, Crick and the Double Helix |
Watson and Crick based their model on Franklin's X-ray diffraction photograph. Their model had a uniform width of 2 nanometers and bases stacked .34 nanometers apart. The width suggested the presence of 2 strands (as Linus Pauling thought) not 3 (as Wilkins initially proposed).
Franklin's X-ray data implied the helix makes a full turn every 3.4 nanometers; because .34 nanometers apart, there are 10 layers in each full turn. At the time Adenine and Guanine were known to be the same type: purines; as well as Thymine and Cytosine: pyrimidines
purine + purine = too wide; pyrimidine + pyrimidine = too narrow; pyrimidine
+ purine = consistent with X-rays
Why?
Adenine
and Thymine have 2 binding sites (hydrogen bonds).
Cytosine
and Guanine have 3 binding sites (hydrogen bonds).
Any
other arrangement would make DNA unstable.
This
complies with Chargaff's Rule.
Next: "Meselson, Stahl and DNA replication."