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DNA fingerprinting is a laboratory procedure that
requires six steps:
1: Isolation of DNA. DNA must be recovered
from the cells or tissues of the body. Only a small amount of tissue -
like blood, hair, or skin - is needed. For example, the amount of DNA
found at the root of one hair is usually sufficient.
2: Cutting, sizing, and sorting. Special enzymes called restriction
enzymes are used to cut the DNA at specific places. For example, an
enzyme called EcoR1, found in bacteria, will cut DNA only when the sequence
GAATTC occurs. The DNA pieces are sorted according to size by a sieving
technique called electrophoresis .
The DNA pieces are passed through a gel made from seaweed agarose (a jelly-like
product made from seaweed). This technique is the biotechnology equivalent
of screening sand through progressively finer mesh screens to determine
particle sizes.
3:
Transfer of DNA to nylon. The distribution of DNA pieces is transferred
to a nylon sheet by placing the sheet on the gel and soaking them overnight.
4-5: Probing. Adding radioactive or colored probes to the nylon sheet
produces a pattern called the DNA fingerprint. Each probe typically sticks
in only one or two specific places on the nylon sheet.
6: DNA fingerprint. The final DNA fingerprint is built by using several
probes (5-10 or more) simultaneously. It resembles the bar codes used
by grocery store scanners.

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