Microbiology terms - G


G+C ratio
In DNA (or RNA) from any organism, the percent of the total nucleic acid that consists of guanine plus cytosine bases.
gamete
In eukaryotes, the haploid cell analogous to sperm and egg, which results from meiosis.
gas chromatography
A chromatographic technique in which the stationary phase is solid while the mobile phase is gaseous samples. The gaseous samples are separated based on their different adsoprtion ability to the solid phase.
gas vacuole
A subcellular organelle, found only in prokaryotes, which consists of clusters of hollow, cylindrical, gas-filled vesicles (gas vesicles).
gas vesicle
The hollow, cylindrical, gas-filled structure in the gas vacuole. It confers ability for gas vacuole to float.
gel
An inert polymer, usually made of agarose or polyacrylamide, used for separating macromolecules such as nucleic acids or proteins by electrophoresis.
gene
A unit of heridity; a segment of DNA specifying a particular protein or polypeptide chain, a tRNA or a rRNA.
gene amplification
see amplification.
gene cloning
The isolation of a desired gene from one organism and its incorporation into a suitable vector for the production of large amounts of the gene.
gene copy number
see copy number.
gene disruption
Use of both in vitro and in vivo recombination to substitute an easily selected mutant gene for a wild-type gene.
gene library
A collection of cloned DNA fragments that contains all the genetic information of a particular organism.
gene therapy
Replacement or augmentation of a dysfunctional gene for medical purposes.
genetic code
The "code" in which information for the synthesis of proteins is contained in the nucleotide seuqence of a DNA molecule (or in certain viruses, of an RNA molecule).
generation time
Time needed for a population to double. See also doubling time.
genetic engineering
The use of in vitro techniques in the isolation, manipulation, recombination, and expression of DNA.
genetic map
The physical arrangement and order of genes on the chromosome.
genetics
The science of studying heredity and variation of organisms.
genome
The complete set of genes present in an organism.
genotype
The precise genetic constitution of an organism. Compare with phenotype.
genus (plural genera)
The first name of the scientific name (binomial); the taxon betwen family and species.
germicide
A substance that inhibits or kills microorganisms.
glycocalyx
General term for polysaccharide components outside the bacterial cell wall. See also capsule and slime layer.
glycolysis
Reactions of the Embden-Meyerhof pathway in which glucose is oxidized to pyruvate.
glycosidic bond
A type of covalent bond that links sugar units together in a polysaccharide.
Golgi complex
An eukaryotic organelle involved in the secretion of certain proteins.
gonococcus
Neisseria gonorrhoeae, the gram-negative diplococcus that causes the disease gonorrhea.
gonorrhea
An acute infectious sexually transmitted disease of the mucous membranes of the genitourinary tract, eye, rectum and throat. It is caused by Neisseria gonorrhoeae.
gram-negative
To describe a prokaryotic cell whose cell wall stains pink (negative) in Gram stain. The cell wall of a gram-negative bacterium contains relatively little peptidoglycan but contains an outer membrane composed of lipopolysaccharide (LPS), lipoprotein, and other complex macromolecules.
gram-positive
To describe a prokaryotic cell whose cell wall stains purple (positive) in Gram stain. The cell wall of a gram-positive bacterium consists chiefly of peptidoglycan and lacks the outer membrane of gram-negative cells.
Gram stain
A differential stain that divides bacteria into two groups, gram-positive and gram-negative based on the ability to retain crystal violet when decolorized with an organic solvent such as ethanol.
green (sulfur) bacteria
Anoxygenic phototrophs containing chlorosomes and bacteriochlorophyll c, cs, d or e and light harvesting chlorophyll.
growth
In microbiology, an increase in both cell number and cellular constituents.
growth factor
Organic compounds that must be supplied in the diet for growth because they are essential cell components or precursors of such components and cannot be synthesized by the organisms themselves.
growth rate
The rate at which growth occurs, usually expressed as the generation time.

Compiled by Tsute Chen, Converted by Ben Hoyt