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easy DNA extraction lab

• Blender
• Medium or large container
• Toothpicks or stirring rod
• Transparent cups(beakeres recommended)
• Salt
• Measuring cup
• Tablespoon
• Liquid soap
• Liver or banana (depending on which one you are going to extract from)
• Coffee filter or paper towels (filter paper recommended)
• 3 Vials or test tubes
• Isopropyl alcohol (rubbing alcohol)
• Funnel
• Wax crayon

1. Using a wax crayon, mark the halfway point of each vial or test tube.
2. The following shows the amount of cell source that you should use:
   • liver: 2-3 inch slice
   • banana: 3/4 or one full banana
3. Chop the liver or banans into coarse pieces.
4. Put the chopped material into the blender.
5. Add 1/2 cup of water to the chopped material.
6. Add one tablespoon of salt to the mixture.
7. Add 1/2 cup of liquid soap to the mixture.
8. Blend for about 10-15 seconds so that the mixture is consistent.
   • Note: you will probably end up with excess blended material in which case you can repeate the experiment (recommended), or you can make a really dusgusting shake (not recommended).
9. Fold a coffee filter or filter element into a cone shape to place in the funnel.
10. Place a cup under the funnel and slowly pour the blended material into the funnel to filter through into.
    • If the mixture is too thick, you can use a metal wire strainer as an alternative filter
11. Pour the filtrate (the stuff in the cup) up to the halfway point in each test tube or vial.
12. Pour the alcohol into each vial until they are almost full.
13. Wait for the DNA to rise to the top of the mixture.
14. A stringy mucousy layer with tiny bubbles will form on the top layer once the DNA has risen.
15. Wow, cool! You've just extracted DNA!
16. Use a toothpick to remove some of the stringy DNA from the top layer and observe it.

Very cool!

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