Thursday Mar. 02,2006
Our cells were subjected to two environment changes. The first change was made after 5 days from effecting the culture and the second one at the end of 8 days from creation of the culture. 1 st passage is carried out on the 10 th day i.e, today.
Composition of DMEM Culture Medium- FBS (Fetal Bovine Serum) :
The cells were to be subjected to a passage today. It is carried out in order to bring further purity. Passage is the displacement of the cells to a larger container from the smaller one. This is carried out because the cells shall require larger space to grow further. The cells arrive into a phase of unwanted differentiation in case there isn't the larger space needed by them. Glutinous cells are thrypsinized in order to approach such an objective. The medium and the cells are taken to a Flacon 15 tube in case the cells are not agglutinated. They are subjected to a centrifugal process and the surface fluid and deposited cells are collected. The deposit is dissolved in a new medium and taken toa larger container. However, 0.25% thrypsin is used for the mesenchymal stem cells agglutinated on the bottom of the container. The fluid on the cells is removed under sterilized conditions and 2ml of PBS buffer is added to the same. PBS is removed and 0.5 –1 CC of thrypsin is added to the cells. They are regularly monitored under a microscope and a medium containing serum (about 4 CC) is added in order to neutralize thrypsin. Then the fluid containing the cells is taken to a larger pipet (flask) and culture medium is added with the same.
The report on the findings and results is incomplete because of the continuation of the operations under the project.
The bone marrow cells after passaging