Monday Feb. 19,2006
The Method For Extraction of Bon-Marrow Cells In A Rats Leg :
The rat is given anaesthesia by using chloroform. The rat is first anaesthesized and it dieswithin 5 minutes.The rat is fixed on a unolith and the abodminal area is shaved in order to avoid the disturbance from the hairs on that part. An incision is made on the leg with a blade and the bone is taken out after.The tissues surrounding the same have been removed; some type of eluant is made available in the cell culture cabin and the bone is cleaned and rinsed in the eluant. The two ends of are cut out and the resulting piece of bone is placed in some other fluid to carry out further cleaning. A solution is put through the bone in question in order to extract bone-marrow cells. The procedure is repeated 3-4 times until as many bone-marrow cells as practicable are extracted. Care should be taken to avoid any overflow of the solution from the piece of bone. The piece of bone shall have a white appearance after the aforesaid procedures and almost all cells shall have been taken into the fluid. The fluid will be placed in Falcon 15 and subjected to a centrifugal procedure so that the cells are deposited. Centrifugal procedure will be at a rate of 1000-1200 rounds per second for 5 minutes.
