Siberian Bouquet
Russian version |
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About the project
Plants are a basis of a life on the Earth. They form
enormous quantity of an organic matter during photosynthesis at interaction
of a solar energy, water and carbonic gas. Thus free oxygen is liberated
which brings to an atmosphere. Its content in the atmosphere of the Earth
is about 21% and is constantly supported at this level. All this oxygen
is a biogenic origin. The matter formed during photosynthesis is the basic
source of existence of herbivorous animals and as a result of predatory
animals and other organisms. Thus the plants are a source of existence
of people. They feed them, dress, treat, give habitation and
fuel. The comprehension of the major role of plants in the life of all
population of the Earth also has pushed us to begin the work an this project.
In this project we’d like to tell you about our researches. Julya
and Asya as the most serious of us summarized materials of researches
for some years during the 2004 summer practice. Those researches had been
done by students of Shelekhov Lyceum. It show the reactions of plants
for pollution of the atmosphere and the soil. Olya and Nikita were engaged
in systematization of plants, collection of stories and legends that are
connected with the plants. Sometimes magic legends had created a bright
impression on them. So they wanted to paint the illustrations for those
legends. Furthermore they had been watching closely for the motion of
a floral clock and for display of a floral barometer since an early morning
till the night. Of course, there is a common thing amount those different
researches. It is the observation of the quiet life of other inhabitants
in our planet. These inhabitants are plants. They can’t tell aloud
about what they are felling. But a kind and careful student's look can
open the secret of their significant silence. Do we behave as a part of
the population of the Earth rightly? What do our neighbours by the planet
feel beside us? What can we do for improving the real situation?
We’d like to answer for these and other questions by our project.
So the main "highlights" of
our project are:
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the original pictures of the plants;
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the original illustrations of the legends about
flowers;
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the photos of the wonderful places of Eastern Siberia;
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the publication of the results of long-term researches
of students of Shelekhov Lyceum;
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and especial the soul of our team enclosed in each
page of the project.
We hope that many people will find something interesting on these pages.
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About us
We live in a small town of Shelekhov and we are studing in Shelekhov
Lyceum. Our town is situated near big Aluminium Plant. We know that our
town is the area of bad ecological situation. But we were born there and
we think that this place is our motherland. We want to improve the situation.
So we should do everything that we can. First course that we can do now
studying in our Lyceum is:
— to learn everything that is connected with living beings’
life in negative ecological conditions
— to analyse facts of experiments
— to do the conclusions that may be used for improving ecological
situation in future.
We are involved in a researching assignment during the summer practice.
This summer practice is led in two places: on the one hand – in
an ecologically dirty territories over the industrial zone; on the other
hand — in an ecologically clear place.
Every year, since 1995, a group of our students has been gone to the settlement
of Arshan. The settlement of Arshan is supposed to be one more pearl of
Eastern Siberia is a mountain balneal resort. It is located far from any
industrial factories. That’s why we research local plants, variety
of species there. There are a lot of species because this place has a
different zoning. We collect stories and legends about the plants and
observe plants’ behaviour during the day. We can find out the time
by a floral clock and forecast the weather by a floral barometer. We test
plants that will be named “background” later. We do it this
way because further we will compare these tests with the tests from urban
districts.
Teachers and students of Shelekhov Lyceum have been engaged in this work
for some years.
A big experiment material has already been saved up. Therefore we want
to tell you about the conclusions of researching. The main conclusion
that has been made during the work is that plants don’t just watch
what happens around them but react to the changes of nature sharply. There
is a different reaction. But for some reasons plants react more often
to people’s activities with pain. Perhaps it isn’t too late
to correct something…
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Location
We live in Asia. Our town Shelekhov is situated in 10 kilometers from
Irkutsk that is the center of Eastern Siberia in Russia. Not far from
our town Lake Baikal is situated. It is the pearl of Russia and the deepest
and cleanest lake in the Earth. The places around the Baikal amaze everybody
by their beauty and richness of nature landscapes. But our town that is
situated in 100 kilometers from the Baikal represents another picture.
Shelekhov was built 42 years ago because of the big construction of Irkutsk
Aluminium plant. The town was named by Gregory Shelikhov who is known
as “Russian Columbus” and who was an Irkutsk merchant and
founder of the first Russian settlements in Alaska.
Aluminium plant rises and increases its power every year. The majority
of population of Shelekhov works in its shops. But the distance between
the town and living areas isn’t enough – only 2.5 km! Unfortunately,
aluminium is made from bauxites and it is a faulty technology. So, in
the same time the plant feeds all people in the town and poisons them.
Building of cleaning constructions doesn’t keep pace with the rise
of production. And beautiful pine-trees forests that have been grown there
gradually are disappearing. It is dolefully to see a grey smog over the
town when you are driving up to it.
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Shelekhov & Arshan
Shelekhov is the town with a high concentration of industrial factories.
There is Factory of ferroconcrete items, Cable plant, Lime factory and
Tractor plant except Irkutsk Aluminium plant. At the same time discharges
in the atmosphere as a result of motor transports’ working and stove
heating are added to them. A pollution level of air in the town is very
high. Average measures of concentration of substances during the year
exceeded rated value of concentration. For example concentration of benzopiren
is 12.9 times as much, of formaldehyde – 6.7 times as much, of fluoride
– from 4 to 8 times as much, of anhydrous hydrogen fluoride –
from 5 to 1.5 times as much and of dust – from 1.3 to 2.7 times
as much.
Arshan Settlement is located in Tunka valley at a distance of 350 km from
Shelekhov. There aren’t any industrial factories. Local population
is Buryats. They think that a valley of the river Kyngarga where Arshan
settlement is situated is a holy place for a religious worship. The fact
is that mineral springs in the river and along its banks are gushing out
from under the earth. So its water helps from many diseases. Not far from
the settlement Dacan is situated. It is a buddhistic monastery. Buddhistic
monasteries are built only in holy places where a powerful source of the
space energy has been discovered. Exactly in that place Arshan settlement
is situated with icy mountain river Kyngarga and hot mineral springs,
violent waterfalls and blue mountain ranges of Eastern Saians stretching
straight to the sky.
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Camp
Our life in the summer camp in Arshan settlement is a real exotic story
in comparison with school weekdays. It is a holy place with the summer
heat and wonderful mountain air. So in that place we take a rest for a
long winter and studying and store up fresh energy and impressions. We
have lived in the camp only for two weeks but have taken a shot of sprightliness
for all year long. Except the rest we work. We have botany, biology and
ecology lessons. Every year we take plants’ tests in the same areas
of meadows, in the forests and foothills. We do it in order to conduct
a longstanding observation. Teachers say that this way we “are involved
into the idea about integrity and dialectics of natural complexes of our
region“ and that we “improve the skills of a field research,
office studies and analysis of materials”. At the same time we find
out a lot of interesting things about plants, legends and stories about
them and possibility to use plants in medicine. It is a grown-up view
to the aims and problems of our camp. But this work is often very fine
and interesting for us!
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Observations
During the summer practice we did not simple gather the plants for researches.
Also we did some natural observations. It was the most interesting part
of the practice Well, it was done like this: the schedule of field observations
was done for the students. Two students had to observe and describe the
life of the forest and field plants for two hours. Since the early morning
till the dark night we could observe the plants. That observations told
us that plant lifetime was being influenced by the definite biorhythms.
And the most definite daytime biorhythms were for the plants with big
and bright petals.
In the same time these plants' flowers opened and closed. This plants’
ability is known for a long time. In the Ancient Grice and Rome special
plants were put in the flower garden. That plants opened and closed their
coronas in different time so they knew the time.
A popular botanist of 18-th century Charles Linney who had studying the
plants for a long time discovered the law of their coronas’ opening
and created a floral clock. That clock was in the town called Upsala in
Sweden. Clock-face of this clock was divided into sections. Every section
had a definite kind of plants that were selected by the time of their
opening. During the sun day floral clock worked very well: every hour
one plant opened its flower, then another plant opened its flower till
the dusk.
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Local plants
There are the rich flora in Eastern Siberia. We tell about some local
plants in the project:
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Floral Clock
We live in a different climate so we ratio our summer Floral
Clock for our region.
What happens on the forest fields and flowering meadows of Eastern Siberia
during the long summer days and short nights?
When the daybreak begins the first plant that opens the flower is a yellow
salsify. Its petals have flashed since 3 to 4 hours in the morning. After
that a blue chicory gets up, then red petals of a poppy appear and sunny
spheres of dandelion and garden sow-thistle present themselves. A bush
of a dog-rose shows its red flowers after some time. By 6 o’clock
blue fields of a flax wake up, fields of a potatoes with white-purple
flowers mote and a carnation break up into many red “coats”.
At 8 o’clock a water-lily and a bindweed swim up and straighten
its petals. At 9 o’clock when the sunlight is everywhere a calendula
and a foalfoot open their flowers.
By the middle of the day a field is full of different colors of plants
and after that the plants that have been opened earlier start to fall
asleep.
The first plant that closes its corona is a salsify. After it is a dandelion.
After 5 o’clock a water-lily swims down the water and the flax field
greens. But till the sunset we can observe the flowers of the dog-rose.
But there are the plants that open at the night.
After the sunset a wild orchid begins to be fragrant. In the dusk a ragged
robin shows its snow-white corona. Its flowers seem to be like the stars,
its sweet-scented flavour is feeling everywhere and it attracts butterflies-pollinators.
Almost every night a flower has a white color and a nice strong spirit.
They are so white because only white flowers are seen by insects-pollinators
through the dark green glass. A strong flavour leads insects to the flower.
So “waking up” and “dream” of plants in a definite
time of the day or night are plants' accomodation to insects-pollinators.
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Barometer
Floral clock has one special feature – it works by its own rhythm.
It shows the time only in a cloudless sunny day and in cloudy days and
before the change of the weather they don’t open or they close too
early.
We have tried to learn this special feature of local plants and discovered
some “barometers” amount them. The flowers of a yellow acacia
waiting for the rain excrete a lot of nectar this flavour attracts bees
and the bees’ swarms go around the bushes. By their buzzing we can
forecast the rainy weather. But in nice days or before the dry weather
an acacia and honeysuckle almost do not excrete nectar. The flowers of
a sow-thistle, a flax and a dandelion do not open in the moisture weather.
Before the long bad weather a clover pulls down its triple leaves.
But there are the plants that forecast the dry weather. For example, before
the hot weather a fern twists its leaves. It is the form of protection
from drying.
Flowers of a cornflower are very sensible for the changes of the weather.
If they opened in spite of the cloudy morning you will be able to wait
for the good weather. A violet is a good barometer. If the flower is open
and it looks at the world by its funny purple eye then the weather will
be good. This plant closes its flower and wilts before the bad weather.
The same thing happens with a daisy. Before the bad weather its flower
bends to surface of the ground very low. Let look at a bindweed. A bindweed
can forecast the weather. A bindweed closes its flowers by the rain and
it opens even at the cloudy weather.
You can see a thistle on litter wastes, in ravines, on precipices and
on pastures. It is an excellent weather foreteller. Before the cloudy
weather the thistle presses its thorns to its bulb densely and the thorns
don’t absolutely prick. When it is the heat it turns up its thorns
from the bulb, so its bulb is pricklier.
The white water-lily is one of the most beautiful our plant. The flower
of white-lily is the original weather station. Sometimes the lily is late
for going out to the surface. If it is the start of nine o’clock
and the water-lily has just started to go out of the water it will be
raining in the latter half of the day. And if the water-lily hasn’t
already gone out of the water a long heavy shower or fall of temperature
will be. You can make a permanent forecast by the leaves of this plant:
if the leaves cover water surface thickly in spring frosts won’t
be.
Waiting for the rain weather rose and dog-rose don’t open their
buds too.
Conifers have one remarkable feature: they lower their branches before
the rain and lift them up before the fair weather. A fir especially shows
it. Centuries-old national practice shows that you have analyzed the whole
row of plants can do permanent weather forecasts.
For example, everybody knows a birch tree. The birch is the most widespread
deciduous sort of trees in Russia. There are a lot of descriptions that
are connected with the birch. All of them have been saved up for many
centuries. Would you like to know what weather will be in the next summer?
If the birch blossoms out its leaves before the alder-tree in spring summer
will be dry. If the alder-tree is faster then the birch cold and rain
will be. If there is a lot of birch sap in spring summer will be rainy.
Do you know that birches tell us about the spring weather in fall? Well,
if the leaves begin to turn yellow from the top the spring will be early.
If the leaves begin to turn yellow from the bottom the spring will be
late. If the leaves begin to turn yellow evenly the spring will be middle.
Birches can forecast the winter weather too. If the leaves haven’t
fallen off yet in the beginning of October snow will be late. If the leaves
fall off on time long thaw will be in the end of January — beginning
of February.
So, plants react to changes of the atmosphere conditions. But if you’d
like to determine coming weather you ought to use some descriptions and
compare them.
Observing on behaviour and life of plants we have learned how to forecast
the weather exactly by hardly visible signs.
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Legends about flowers
We have made the collection of
legends about plants which are in our edges.
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Researches
We would like to acquaint you with the content of the research works
that have been made by students of Shelekhov Lyceum. We had gathered materials
for these researches during the summer practice that were processed and
analyzed later. The conclusions were done by the results of analyses corresponding.
Julia generalized the results of three research works that had one general
problem — the reaction of plants to the environmental pollution.
The principle of the researches in two works was the same: the birch leaves
and the mossy bodies had been gathered, dried up and then the content
of zinc and manganese was defined in samples. The principle of the research
in the third work is the definition of pine needles suffering in percents.
The samples for the experiences had been undertaken in a pure ecological
zone (Arshan). We called them "the background samples", and
some samples were gathered on several sites of Shelekhov. All of these
samples were compared to the laboratory samples. The method used for calculation
of the pine needles suffering was the most of the simple. This method
is a visual analysis. But it was more difficult to definite the content
of microelements because the special equipment was necessary.
And why was that principle laid down in a basis of the researches?
Many biologists and chemists of the past researched living beings and
tried to find a special substance or “living force” tha t
causes the organism functioning. But no substance, even any instructions
on it were found. Scientists found out that organisms consist of the same
chemical elements that form the air, water, rock and soil minerals.
There are 80 chemical elements in the mixture of living organism. The
organism needs some elements in a big quantity (they are called macro-elements)
and other elements in a negligibly small quantity (they are called micro-elements).
It is important to know the role of each element in forming of a living
organism vital function.
Frey Basling had paid attention to the fact that chemical elements necessary
for the plants take a different position in periodic system. Further researches
showed that chemical element life need isn’t determined only by
the position in the periodic system but it determines by a whole row of
characteristics such as an ionization potential, ionic radius, polarizability
and ect. It is clear that element toxicity increases according to the
increase of its atomic mass. Content of these elements is usually too
little in the organism.
Microelements are the elements that content in living organisms doesn’t
exceed 10%. Microelements take part in breathing, photosynthesis, protein
synthesis, protein and lipid exchanges, humus synthesis and other processes.
On average content of Zn in the plant ashes is 10 and in the dry plant
is from 15 to 70 mg/kg. A number of elements in soil doesn’t exceed
65 mg/kg and assimilated by plants is from 0.03 to 20 mg/kg.
Biological absorption factor (BAF) of Zn is 10. A feature of this element
is its low chemical activity. That’s why it is partly involved into
the second circulation. Zinc takes part in the intracellular regulation
in plants.
The average content of Mn in the plants is 10 mg/kg and BAF swings from
0.1 to 100. In the plant cell maximum of Mn can be find in its cytoplasm.
Manganese is a part of many ferments and chlorophyll.
And it stirs their formation to activity. Manganese takes an active part
in breathing, photosynthesis, synthesis, nitric and nuclein exchanges.
The activity of Mn in different physiological and biochemical processes
isn’t determined only by its quantity in the plant but it is determined
by the ratio of Mn to other chemical elements that take part in these
processes. The ratio of Mn to Fe and Cu is especially important.
Iron comes to the plant as a part of chelates. It is has a bivalence condition.
High concentrations of Fe (II) may influence on the plant toxically. Iron
acidified and precipitates over Mn. But this process proceeds by a special
ratio of these elements. If this ratio gets broken the following things
happen:
- A lack of the content of Mn in the plant brings to an excessive Fe (II)
concentration. This accumulation of Fe (II) can reach a toxic concentration.
- Fe (II) is transferred into Fe (III) with the help of Mn excess. Then
Fe (III) accumulates in the form of organic-phosphoric Fe and a plant
begins to suffer from the lack of Fe (II).
Copper influence on the content of manganese similarly. That cooperation
is visible not only in the plants but in the soil. Manganese takes part
in bud-formation. Soil horizons that are fortified by Fe are particularly
illuvial and they contain the increased quantity of Mn.
What is need for normal organism functioning?
1. At first, the determination of microelements concentration in habitat.
2. Secondly, definite ratio of absorbed elements
3. Thirdly, definite forms of compound where microelements in habitat
are situated.
Non-observance of these demands brings to violation of different biochemical
processes. Increased or decreased concentration of microelements in habitat
may cause the endemics.
Why was maintenance of zinc and manganese exactly determined in samples
of plants?
Zinc also plays an important role in a plant life, that’s why increase
or decrease of it causes different diseases of plants. Decrease of Zn
results in delay or stopping of many plants growth. Small-leaves, rosette
and defoliation are observed on trees. Chlorosis is observed on maize,
leaves pairing – on tomatoes, leaves mottling – on citrus
plants.
Shortage of Mn in soils brings down growth and development of the plant.
At the same time iodine assimilability of many plants is decreased. Tannin
formation of tanning plants is decreased, mottling and odor nuisance of
oats and spottiness of beans become apparent. Many plants amass iron under
the deficit of Mn and the content of Fe is decreased under the excess
of Mn (that brings to chlorosis).
In a couple of “plant-soil” is observed the dependence between
the content of chemical elements in the plants and in the soils. When
there is a little quantity of the element in the soil plant absorbs all
available quantities of this element forms in spite of the fact that the
quantity of the element in the plant is decreased.
Under the optimal content of the element chemical composition of the plants
mainly depends on the plant biological features and physiological role
of that element.
This way a geochemical surrounding isn’t the limitative factor.
So regulation machinery starts working in the plants. The content of an
absorbent element is determined by a need of the physiological processes
realization. It is clear that climatic conditions especially temperature
and moistening influence on the plant’s absorb of the elements and
therefore on the chemical composition. For example, in the northern regions
the plants contain more Cu, Mn, Zn than here.
A chemical composition of the plants depends on the technogenic processes
activity. A technogenic type of the elements migration appeared as a result
of human activity isn’t submitted to nature factors but socio-economic
laws and it presents a variety in accordance with its origins. Also it
includes the transference of products of natural raw materials on long
distances.
Fully unconsumed raw materials, solid and gaseous waste products of industry
come to the atmosphere and solid and liquid waste products – to
reservoirs. It begins to a sharp re-distribution of chemical elements
that is called on a violation of biogeochemical circulation of such elements
as C, B, P, Pb, Cu, Zn, Fe, Hg, Ag, Mn.
Distribution of chemical elements as a result of human economic activities
is uneven that bring to a high concentration of chemical elements in local
places with their following “diffusion” in more wide natural
habitats. In other words technogenic anomalies that are connected with
the work of industrial centres are formed.
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Object & Metod
Objects of researches
Students of Lyceum learned the content of zinc and manganese in the birch
leaves and in mosses that were collected on the areas of Shelekhov and
Arshan by theyself. Other students studied the needles of the pine-trees
as the indicators of the pollution of the environment. Why were these
the objects chosen?
1. We compared to per cents content of zinc and manganese in the clean
birch leaves with per cent of content of zinc and manganese content in
the dirty birch leaves gathered at the suburbs of Shelekhov and at Arshan
(background sample). We find out that the birch leaves growing in the
fields near the Irkutsk Aluminium plant contain zinc in twice more and
manganese almost in ten times than the background leaves contain. What
are the reasons of that fact?
2. Mosses belong to the plants that are the most sensitive for the pollution
of habitat. That’s why they may be chosen for the object of analysis.
3. Xeromorphos needles of pine-tree have low gas resistance for some reasons:
a. A needle has a long lifetime. It is constantly
exposed by the influence of gases during its lifetime.
b. A needle has a high sickness rate in comparison with deciduous species.
Process of needle forming and rise brakes because of
pollutant influence. Because of it needle lifetime decreases to 2-3 years
and in individual cases – to one year while it reaches to 6-9 years
in young inviolate pine forests. Chlorosis and necrosis of assimilated
plant organs belong to outside changes that are the results of man-caused
pollutant influence. These features are of a big interest of diagnostic
counse.
The plants that have been chosen for research are members of different
departments like Mosses, Gymnospermae and Angiospermae. That gives us
a possibility to learn not only the chemical composition of these plants
but their taxonomy. That’s why our researches have a complex character.
In order to perennial observations for condition of plant components of
biogeocenoses five monitoring areas (or sub territories) were placed.
They had a square of 400 square meters and identical plant dominants and
soils. Three control areas were placed around Shelekhov and two background
areas – around Arshan settlement. On these areas total geobotanical
and soil descriptions were done, samples of plants and soils were taken
for chemical analyses. Common sampless were prepared by chair of botany
and analytical researches were done by chair of physical methods of analysis
of Irkutsk State University.
Method of researches of birch leaves and mosses
Roentgen-spectral fluorescent method of analysis (RSFA) was used for the
determination of the chemical composition of the mosses and content of
the chemical elements in the birch leaves.
Distinguishing feature of a modern RSFA is that it is used for the determination
of elements in the biological objects and for the control of the pollution
of the environment. In comparison with other physical methods of analysis
(such as an atomic absorptive, an emissive spectral, a neutron activation)
when apparatus with the energy spectrum decomposition is used RSFA has
the following advantages:
1. A possibility to determine a big number of elements at the same time:
2. A conservation of the research sample’s materials that gives
us the possibility for its second analysis by different methods:
3. A slight man-hours for analysis, an easy spectrum treatment, a possibility
to use computers for the calculation of the concentration:
4. This method falls for automation easily.
The last two advantages are the most important for the realization of
mass analyses.
Preliminary researches have showed that Mn, Fe, Zn, Nb Pb, Sr in the mosses
may be determined by RSFA. So the aim of the present work is to elaborate
the system of the Roentgen-fluorescent determination of Zn and Mn in the
mosses that have a high rate of content of Zn and Mn rather than the content
of other listed elements.
Five samples of mosses and birch leaves were selected for the realization
of the researches in different places. The first two samples were selected
in Arshan settlement. The second sample — at the suburb of Shelekhov
at a distance of 3 km from the industrial zone, the third sample was selected
at a distance of about 6 km from the industrial zone and the forth sample
— at a distance of 0.5 km from Irkutsk Aluminium Plant. Samples
that had been taken in Arshan were named as a background. The samples
that have been selected at the suburb of Shelekhov were named as control.
In other words not only influence of nature facts were estimated by them
but also the influence of environment for the content of Zn and Mn.
Clean and dirty birch leaves and moss were analyzed independently after
another. It was done for determination of the ways of Zn and Mn penetration
in plant’s organism (from the soil or the atmosphere). Each sample
of the clean and dirty birch leaves was analysed four times.
Apparatus
The researches were led by the roentgen fluorescent spectrometer VRA-30
that is produced by the firm “Carl Zeiss”. This spectrometer
has one scanning channel and is completed by three roentgen pipes with
different anodes (tungsten, rhodium and chrome). X-radiation decomposes
into the spectrum using crystal-analysers LiF(200), LiF(220), PE, ADP,
KAP, Si by Soller method.
Scintillation and flowing proportional counters are used for the registration
of the radiation. The first one is used for K- radiation measuring of
elements that have numbers Z>25 and for the radiation measuring of
L-series of the elements with Z>60. The second one is used for X-radiation
measuring of the elements those are from 9K to 29Cu and for K-radiation
measuring of the elements with Z<60. All process of the regulation,
measuring and inside control of the device are led by inside microcomputer.
During the experiments K-radiation activity was being registered under
the conditions that the tension of the roentgen pipe was 40 kV, current
strength was 40 kA, exposition lasted for 60 seconds and crystal-analyser
LiF(200) and scintillation detector were used. K-lines of Zn and Mn were
the analytical lines.
Method of research of pine-tree needles
The system that had been published in the book “Ecological monitoring”
by F. Snakin (Moscow, 1996) was used for needles research. Observations
had been continued for 3 years (2000, 2001, 2002). 10 branches of pine-tree
that was 2 years old were selected from 10 trees on each area then general
number of needles, numbers of healthy and injured needles were calculated.
There were chosen 3 classes of needles injury (or necrosis):
1. Needles without spots or healthy,
2. Needles with a few small spots,
3. Needles with a lot of black and yellow spots and completely yellow
needles (or dried needles).
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Work
Preparation of sample of birch leaves for the analysis
Selected birch leaves had been dried with the limited sunbeam hit before
the laboratorial samples were created. After that the sample was divided
into two parts. The first part had being dried in desiccator till moisture
completely removed. That part was called a clean leaf. The second part
had left without changes. This part was called a dirty leaf. Then dried
leaves were cut into very small parts about 1 mm with the pair of scissors.
The six sample’s masses of 5 grammas were taken from the material
of the sample after cutting and another mass was left without changes.
And the others were being grinded with spirits into the agnatic mortar
during 5, 10, 15, 20 and 30 minutes accordingly.
The radiators were prepared by pills’ pressing at a substrate of
boron acid in a special press-form.
Two grammas of materials were taken for 1 pill. So from each sample mass
2 pill-radiators were gotten. After that Co-lines of Zn and Mn activity
was measured from each pill.
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Results
Birch leaves research results
Middle analysis results and their trusting intervals that have been calculated
if probability is P = 0,95 are shown in the first table.
First table. Birch leaves analysis results:
Number of sample |
Place of selection |
Content of Zn, mg/kg |
Content of Mn, mg/kg |
Dirty |
Clean |
Dirty |
Clean |
1 |
Arshan |
91 ± 3 |
82 ± 3 |
1010 ± 60 |
840 ± 50 |
2 |
Shelekhov, a distance of 3 km from Irkutsk Aluminium Plant |
80 - 3 |
74 - 3 |
304 ±18 |
287 ± 17 |
3 |
Shelekhov, a distance of 6 km from Irkutsk Aluminium Plant |
94 ± 3 |
90 ± 3 |
100±6 |
68 ± 4 |
4 |
Shelekhov, around Irkutsk Aluminium Plant |
211 ± 5 |
182 ± 7 |
224±13 |
218 ± 13 |
| 5 |
Laboratory sample |
87 ± 3 |
1000± 60 |
It is clear from the table that the second and third
samples content of Zn in the birch leaves (at a distance of 3 and 6 km
from the industrial zone) is similar to the background sample content
(in Arshan). The second sample contains Zn too less than the first sample.
It is clear from these data that technogenesis processes don’t influence
on the content of Zn in the birch leaves in this region.
But the sample of the birch leaves that was gotten in the immediate nearness
from Irkutsk Aluminium Plant contains Zn twice as much as the background
sample. So the increase of the content of Zn is the technogenesis processes
action. The results of the clean and dirty leaves essentially differ from
the results of each other. It shows that the pollution of Zn comes from
the soil and through plant’s stomas with the dust. It needs to say
that a standard laboratory sample of birch leaves that was created at
Irkutsk geochemical institute contains Zn as much as the background sample.
The accumulation of Zn in the birch leaves happens because some intracellular
processes go on.
May-be the increase of use of Zn is the result of pH level changes in
the soil or if some elements or compounds are more or less than it is
necessary.
Determining the content of Mn in the birch leaves we have noticed that
it is a sudden decrease of content of Mn in all samples that have been
gotten at the suburbs of Shelekhov in comparison with background (or Arshan)
samples.
The content of Mn in Arshan sample conforms to the laboratory sample.
So we can say about the defect of Mn in the Shelekhov's samples. Even
more ratio of content of this element in clean leaves to content of it
in dirty leaves allows us to say that Mn comes to the plant through the
soil by rootage. Only the second sample is because its analysis results
of clean and dirty leaves differ from each other. The similar exclusion
is existed the Arshan birch.
The samples of clean and dirty leaves that had been selected in different
areas of Shelekhov and Arshan were analyzed. It was found out that the
content of Zn in two Shelekhov's samples doesn’t differ from the
background (Arshan) a lot. But the exclusion from it is the fourth sample
(Shelekhov, Irkutsk Aluminium Plant) that has the content of Zn twice
as much as in the background sample.
The content of Mn in Shelekhov's samples of birch leaves is from 3 to
10 times as less as in the background sample. Probably the technogenic
process influence is the cause of it.
There are some hypotheses about the reasons of under review fact. The
content of Zn and Mn in clean and dirty leaves doesn’t differ from
each other a lot. So it points at the fact that these elements mainly
come to the plant through the soil by rootage.
Mosses research results
A group of mosses samples were analyzed by developed Roentgen-spectral
fluorescent method (RSFA) system of determination of the chemical composition.
Analysis results are listed in the second table.
Second table. The results of analysis samples of mosses:
| Number of sample |
Place of selection |
Kind of moss |
Content |
| Zn |
Mn |
| 1 |
Arshan, 1 area |
Rhytidum rugosum |
0,0029±0,0003 |
0,049±0,006 |
| 2 |
Arshan, 2 area |
0,0027±0,0003 |
0,047±0,005 |
| 3 |
Arshan, 1 area |
Hylacomium splendens |
0,0032±0,0004 |
0,048±0,006 |
| 4 |
Shelekhov, 2 area |
0,0041±0,0005 |
0,017±0,002 |
| 5 |
Arshan, 1 area |
Dicranum undulatum |
0,0023±0,0003 |
0,022±0,002 |
| 6 |
Shelekhov, 1 area |
Pulaisia polyantha |
0,036±0,006 |
0,27±0,04 |
| 7 |
Shelekhov, 2 area |
0,0068±0,003 |
0,015±0,002 |
| 8 |
Shelekhov, 2 area |
Rhytidiadelphus triguetrus |
0,0098±0,0016 |
0,056±0,009 |
The second table information shows that the content of Zn and Mn in the
mosses that were selected in the first area in Shelekhov (sixth sample)
in other words in the industrial zone is 5 and 18 times as much accordingly
than the content of those elements in mosses that were selected in the
second area in Shelekhov (seventh sample). But those mosses belong to
the same kind. In comparison with the samples of mosses of different kinds
which were selected in other areas the content of Zn and Mn in the sixth
sample is from 4 to 15 times as much. Furthermore preliminary researches
had showed that mosses were selected in the first area in Shelekhov contain
Ni, Fe and Pb from 1.5 to 2 times as much as the other mosses. It shows
the strong industrial zone pollution of heavy metals. Probably the source
of that pollution is Cable Plant.
It needs to increase a number of analyzable samples and to extend the
group of determinate elements in particular to develop the systems of
Roentgen-spectral determination of Pb, Fe and Ni for the final conclusion.
At the same time it needs to find out the ways of heavy metals penetration
into mosses that will be achieved by the analyses of clean and dirty samples.
Pine-tree needles research results
Research results are listed in the following third table:
Number |
Place of selection |
1 (healthy) % |
2 (with spots) % |
3 (dried) % |
1 |
Shelekhov, 2000 |
61,2 |
33,5 |
5,3 |
2 |
Arshan, 2000 |
86 |
12 |
2 |
3 |
Shelekhov, 2001 |
66 |
31 |
3 |
4 |
Arshan, 2001 |
90 |
9,5 |
0,5 |
5 |
Shelekhov, 2002 |
58,9 |
39,8 |
1,3 |
6 |
Arshan, 2002 |
92,1 |
7,6 |
0,3 |
Many researches established that appearance of more than 20 percent of
the damaged needles is evidence of serious forest ecosystem violations.
So our researches confirm that percent of defective needles in Arshan
is contained in borders of the natural physiological process and climatic
influence on needles (from 6 to 9 %). But the percent of the defective
needles in the districts of Shelekhov is 30 – 50 % that shows a
high rate of forest ecosystem violation.
Unlike 2000 and 2001 seasons in 2002 summer the system of the composition
of the variation monitoring areas rows was used that concluded in monitoring
areas established according to approach to or moving off from the pollution
source. In that way 5 areas were placed in the southern direction from
the industrial zone. The first area was at a distance of 15 km from the
industrial zone, the second area was at a distance of 10 km from the industrial
zone, the third area was at a distance of 7 km, the fourth area was at
a distance of 6 km, the fifth area was at a distance of 4.5 km from the
industrial zone.
Information about these areas forms the following table:
|
|
1 area |
2 area |
3 area |
4 area |
5 area |
1 |
healthy |
69,6 |
64,8 |
49,3 |
53 |
58 |
2 |
with spots |
29,8 |
34 |
46,4 |
47 |
42 |
3 |
dried |
0,6 |
1,2 |
4,3 |
|
|
It is clear from the table that a number of needles with spots increases
from 29% to 42% according to the approach to the industrial zone.
The needles are especially sensible for the high concentration of the
compound of sulfur, fluorine and heavy metals.
So we can come to the conclusions:
1. A high concentration of toxic elements of industrial aero-discharges
is amassed in the needle. That fact affects the surface changes.
2. A number of defective needles is directly proportional to the concentration
of sulfur, sulphur dioxide and heavy metals in the air.
3. Visual assessment of the atmosphere pollution is possible by the ratio
of healthy and defective needles to the general number of the needles
in the branch.
Top |
Test
We suggest you to define independently a level of your
awareness about the materials of our site. This test does not assume an
estimation. There can be some productive answers in each question.
Our test
1. What do you know about pine-tree needles?
| |
They change every year; |
| |
They live from 2 to 3 years; |
| |
They live from 6 to 9 years; |
| |
They live during the plant’s life; |
| |
They live more than 10 years. |
2. Double fertilization is a characteristic of…
| |
mosses; |
| |
ferns; |
| |
gymnospermae; |
| |
flowering plants; |
| |
algae. |
3. Mosses are plants that…
| |
have a body in the form of thallus; |
| |
have a root, a stalk and a leaf; |
| |
reproduce by spores; |
| |
are fertilized in water; |
| |
have a flower with easy perianth. |
4. Which of the following plants belongs to gymnospermae?
| |
A tulip; |
| |
A juniper; |
| |
A maple; |
| |
A larch; |
| |
A pine-tree. |
5. Mosses belong to bioindicators because…
| |
They are capable to live in negative ecological conditions; |
| |
They are capable to absorb different substances from environment; |
| |
They indicate pollution of atmosphere and ground; |
| |
They live in the depleted grounds; |
| |
They die by the air pollution. |
6. A birch is an angiosperm plant because…
| |
Its leaves have the cellular nervation; |
| |
Its seeds are inside the fetus; |
| |
It has a flower that is an organ of sexual reproduction; |
| |
It has bright and attracted attention of insects flowers; |
| |
It has the double fertilization. |
7. An indicator of clean air is:
| |
A poplar; |
| |
A larch; |
| |
A lichen; |
| |
A moss; |
| |
A pine-tree. |
8. Plant cells’ membrane lets pass through:
| |
Water; |
| |
Gases; |
| |
Fats; |
| |
Dust; |
| |
Trace elements. |
9. Plant that can absorb pollutant gases from habitat and clean the
air is:
| |
A lettuce; |
| |
An embalmed poplar; |
| |
A pine-tree; |
| |
A clover; |
| |
A yellow acacia. |
10. Firs and pine-trees as other conifers pick out special volatile
matter:
| |
Phytonzides; |
| |
Pheromones; |
| |
Freons; |
| |
Dioxins; |
| |
Poisons. |
11. Arrange plants in order of their bloomings in floral clock:
| |
Foalfoot, dog-rose, salsify, batata, sow-thistle; |
| |
Salsify, dog-rose, foalfoot, batata, sow-thistle; |
| |
Dog-rose, foalfoot, sow-thistle, salsify, batata; |
| |
Salsify, batata, foalfoot, sow-thistle, dog-rose; |
| |
Foalfoot, batata, sow-thistle, salsify, dog-rose. |
12. Why is the worldly wisdom “everything is good fairly”
right for plants too:
| |
Definite concentration of is necessary for the normal organism
functioning; |
| |
Definite ratio between absorbed trace elements is necessary
for the normal organism functioning; |
| |
Definite forms of joining that have trace elements in habitat
is necessary for the normal organism functioning; |
| |
Optimal meaning of abiotic factors is necessary for the normal
organism functioning; |
| |
A plant stops developing normally by a plenty or one factor. |
13. What is observed by the shortage of Zinc of a majority of plants:
| |
Delay and stopping of growth; |
| |
Small-leaves, rosette and defoliation – of trees; |
| |
Chlorosis – of maize; |
| |
Leaves’ pairing – of tomatoes; |
| |
Leaves’ mottling of citrus plants. |
14. What ecological factors have an influence on plants’ life:
| |
Moisture; |
| |
Wind; |
| |
Light; |
| |
Air; |
| |
Features of ground. |
15. What affects on forming of plants’ communities:
| |
Climate; |
| |
Animals; |
| |
Ground type ; |
| |
Relief; |
| |
A human. |
16. Can me say that succession is a process of:
| |
Reconstruction of burnt forest; |
| |
Obliteration earlier lifeless sands; |
| |
Plants’ settling on the roof of village house; |
| |
Plants’ settlement of the railway embankment; |
| |
Obliteration of cutting. |
17. What plants dominate in the forest plants’ community:
| |
Trees; |
| |
Bushes; |
| |
Lichens; |
| |
Perennial herbs; |
| |
Mosses. |
Top |
Sources
Literature:
Busik V.V., Vodopyanova N.S., Ivanova M.M.Flora of Central Siberia.
- Novosibirsk: Nauka, 1979.
Life of plants, ed. Fedorov A.A.- M.: Prosveschenie, 1980.
Biology: ed. Cheremushkina V.A. - Novosibirsk: Nauka, 2002.
Grin N., Staut U., Teylor D. Biology. T.I.- M.: Mir, 1966.
Ivlev A.M. Biogeochemistry.- M.: High School, 1986.
Nebel B. Science of ecology. T. 2.- M.: Mir, 1993.
Ponomarev V.D. Analytic chemistry.- M.: High School, 1982.
Losev N.F., Smagunova A.N. The basis of Roentgen-spectral fluorescent
analysis.- M.: Chemistry, 1982.
Smagunova A.N., Tarasenko SV., Bazykina E.N., Karpukova O.M. Roentgen-fluorescent
analysis in ecology // Journal of analytic chemistry.-1979.- T.34,
N 2.- S 388-397.
Belochinskaya L.I., Mugnueva V.T. Influence of pollutants on physiological
and biochemical characteristic of ancient plants.- Tomsk.
Legends about flowers.
Bazina I.G., Gamzikova O.V., Biryukova E.V. Dependence of the maintenance
of zinc and manganese in leaves of a birch from an environment. Research
work, Shelekhov Lyceum, 2000.
Karpov O.A., Rasputina N.V., Gruzdeva A.N. Development of a technique
Roentgen-fluorescent definitions of zinc and manganese in mosses.
Research work, Shelekhov Lyceum, 2001.
Efimushkin A.A. Needles of a pine ordinary as the indicator of a
degree of environmental contamination of a zone of influence of Open
Society "IrkAZ-Sual". Research work, Shelekhov Lyceum, 2002.
Links:
Science and Plants for Schools
Aboutflowers.com
Redbook of Irkutsk region
Moscow State University Botanical Server
Plants for People
Sacred Earth
Talking Flowers - UK flower delivery
MEANINGS & LEGENDS OF FLOWERS
Tunka's Alps
legends about flowers
Top
|
Credits
The team of Shelekhov Lyceum "Flowers of Life"
consists from the students: Julia, Asya, Olga, Nikita;
and the coachs: Evgenia, Elena.
Julia: the systematization and processing of materials of the
summer practice, English translation, typing.
Asya: the search and processing of legends about flowers, English
translation, typing.
Olga: painting.
Nikita: web-design, Java-Script, Flash-movies, painting.
Evgenia: the general manager.
Elena: the biologist consultant.
In this project photos that had been done by Elena Karpova
and Yuri Scheogolev were used by their approbation. Our
team expresses thanks of everybody who upholded us in the work on this
project. Also we thank Paramonova Natalia, an English
teacher, for consultation in English.
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