Russian

Researches

We would like to acquaint you with the content of the research works that have been made by students of Shelekhov Lyceum. We had gathered materials for these researches during the summer practice that were processed and analyzed later. The conclusions were done by the results of analyses corresponding. Julia generalized the results of three research works that had one general problem — the reaction of plants to the environmental pollution.
The principle of the researches in two works was the same: the birch leaves and the mossy bodies had been gathered, dried up and then the content of zinc and manganese was defined in samples. The principle of the research in the third work is the definition of pine needles suffering in percents. The samples for the experiences had been undertaken in a pure ecological zone (Arshan). We called them "the background samples", and some samples were gathered on several sites of Shelekhov. All of these samples were compared to the laboratory samples. The method used for calculation of the pine needles suffering was the most of the simple. This method is a visual analysis. But it was more difficult to definite the content of microelements because the special equipment was necessary.
And why was that principle laid down in a basis of the researches?
Many biologists and chemists of the past researched living beings and tried to find a special substance or “living force” tha t causes the organism functioning. But no substance, even any instructions on it were found. Scientists found out that organisms consist of the same chemical elements that form the air, water, rock and soil minerals.
There are 80 chemical elements in the mixture of living organism. The organism needs some elements in a big quantity (they are called macro-elements) and other elements in a negligibly small quantity (they are called micro-elements). It is important to know the role of each element in forming of a living organism vital function.
Frey Basling had paid attention to the fact that chemical elements necessary for the plants take a different position in periodic system. Further researches showed that chemical element life need isn’t determined only by the position in the periodic system but it determines by a whole row of characteristics such as an ionization potential, ionic radius, polarizability and ect. It is clear that element toxicity increases according to the increase of its atomic mass. Content of these elements is usually too little in the organism.
Microelements are the elements that content in living organisms doesn’t exceed 10%. Microelements take part in breathing, photosynthesis, protein synthesis, protein and lipid exchanges, humus synthesis and other processes.
On average content of Zn in the plant ashes is 10 and in the dry plant is from 15 to 70 mg/kg. A number of elements in soil doesn’t exceed 65 mg/kg and assimilated by plants is from 0.03 to 20 mg/kg.
Biological absorption factor (BAF) of Zn is 10. A feature of this element is its low chemical activity. That’s why it is partly involved into the second circulation. Zinc takes part in the intracellular regulation in plants.
The average content of Mn in the plants is 10 mg/kg and BAF swings from 0.1 to 100. In the plant cell maximum of Mn can be find in its cytoplasm. Manganese is a part of many ferments and chlorophyll.
And it stirs their formation to activity. Manganese takes an active part in breathing, photosynthesis, synthesis, nitric and nuclein exchanges. The activity of Mn in different physiological and biochemical processes isn’t determined only by its quantity in the plant but it is determined by the ratio of Mn to other chemical elements that take part in these processes. The ratio of Mn to Fe and Cu is especially important.
Iron comes to the plant as a part of chelates. It is has a bivalence condition. High concentrations of Fe (II) may influence on the plant toxically. Iron acidified and precipitates over Mn. But this process proceeds by a special ratio of these elements. If this ratio gets broken the following things happen:
- A lack of the content of Mn in the plant brings to an excessive Fe (II) concentration. This accumulation of Fe (II) can reach a toxic concentration.
- Fe (II) is transferred into Fe (III) with the help of Mn excess. Then Fe (III) accumulates in the form of organic-phosphoric Fe and a plant begins to suffer from the lack of Fe (II).
Copper influence on the content of manganese similarly. That cooperation is visible not only in the plants but in the soil. Manganese takes part in bud-formation. Soil horizons that are fortified by Fe are particularly illuvial and they contain the increased quantity of Mn.
What is need for normal organism functioning?
1. At first, the determination of microelements concentration in habitat.
2. Secondly, definite ratio of absorbed elements
3. Thirdly, definite forms of compound where microelements in habitat are situated.
Non-observance of these demands brings to violation of different biochemical processes. Increased or decreased concentration of microelements in habitat may cause the endemics.
Why was maintenance of zinc and manganese exactly determined in samples of plants?
Zinc also plays an important role in a plant life, that’s why increase or decrease of it causes different diseases of plants. Decrease of Zn results in delay or stopping of many plants growth. Small-leaves, rosette and defoliation are observed on trees. Chlorosis is observed on maize, leaves pairing – on tomatoes, leaves mottling – on citrus plants.
Shortage of Mn in soils brings down growth and development of the plant. At the same time iodine assimilability of many plants is decreased. Tannin formation of tanning plants is decreased, mottling and odor nuisance of oats and spottiness of beans become apparent. Many plants amass iron under the deficit of Mn and the content of Fe is decreased under the excess of Mn (that brings to chlorosis).
In a couple of “plant-soil” is observed the dependence between the content of chemical elements in the plants and in the soils. When there is a little quantity of the element in the soil plant absorbs all available quantities of this element forms in spite of the fact that the quantity of the element in the plant is decreased.
Under the optimal content of the element chemical composition of the plants mainly depends on the plant biological features and physiological role of that element.
This way a geochemical surrounding isn’t the limitative factor. So regulation machinery starts working in the plants. The content of an absorbent element is determined by a need of the physiological processes realization. It is clear that climatic conditions especially temperature and moistening influence on the plant’s absorb of the elements and therefore on the chemical composition. For example, in the northern regions the plants contain more Cu, Mn, Zn than here.
A chemical composition of the plants depends on the technogenic processes activity. A technogenic type of the elements migration appeared as a result of human activity isn’t submitted to nature factors but socio-economic laws and it presents a variety in accordance with its origins. Also it includes the transference of products of natural raw materials on long distances.
Fully unconsumed raw materials, solid and gaseous waste products of industry come to the atmosphere and solid and liquid waste products – to reservoirs. It begins to a sharp re-distribution of chemical elements that is called on a violation of biogeochemical circulation of such elements as C, B, P, Pb, Cu, Zn, Fe, Hg, Ag, Mn.
Distribution of chemical elements as a result of human economic activities is uneven that bring to a high concentration of chemical elements in local places with their following “diffusion” in more wide natural habitats. In other words technogenic anomalies that are connected with the work of industrial centres are formed.

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